![]() ![]() In addition, 0.1% GSE and 0.1% G+P exerted a persistent inhibitory effect on biofilms. As a result, the treatment with 1% GSE for 5 min almost completely eliminated the biofilm formed on the resin whereas, the treatment with 0.1% GSE, Polident, and 0.1% G+P for 5 min showed a statistically significant inhibitory effect on biofilms. Some specimens of acrylic resin were immersed in each solution for 7 days, and changes in surface roughness (Ra), Vickers hardness (VH), flexural strength (FS), and flexural modulus (FM) were evaluated. After incubation, the persistent effect was assessed by CFU assay. In order to assess the persistent cleansing effect, the discs treated with each solution for 5 min were aerobically incubated in Yeast Nitrogen Base medium for another 24 h. After the treatment, the biofilm on the discs was analyzed using a colony forming unit (CFU) assay, fluorescence microscopy, and scanning electron microscopy (SEM). ![]() The discs with the biofilm were treated with each solution for 5 min at 25☌. Discs of acrylic resin were prepared, and the biofilm of C. GSE solution diluted with distilled water to 0.1% (0.1% GSE) and 1% (1% GSE) and solutions with Polident® denture cleansing tablet dissolved in distilled water (Polident) or in 0.1% GSE solution (0.1% G+P) were prepared as cleansing solutions. albicans) formed on denture-base resin and the influence of GSE on the mechanical and surface characteristics of the resin. This study aimed to investigate the cleansing effects of grapefruit seed extract (GSE) on biofilms of Candida albicans ( C.
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